Towards Single Molecule Protein Sequencing

Departments: Mechanical Engineering and Materials Science
Time: Wednesday, March 28, 2018 - 2:30pm - 3:30pm
Type: Seminar Series
Presenter: Derek Stein; Associate Professor of Physics, Brown University
Room/Office: Room 107
Location:
Mason Lab
9 Hillhouse Avenue
New Haven, CT 06511
United States
See map: Google Maps

Department of Mechanical Engineering & Materials Science Seminar

Derek Stein
Associate Professor of Physics
Brown University

“Towards Single Molecule Protein Sequencing”

We are developing a single molecule protein sequencing technology that aims to combine the advantages of mass spectrometry with those of nanopores. The basic idea is to cleave the individual amino acids from a protein molecule as they transit a small hole in sequence, and then identify each one by determining its mass-to-charge ratio in a mass spectrometer. In this talk, I will describe nanopore sensors, our protein sequencing concept, the development of a prototype nanopore mass spectrometer, and the results of studies of the transfer of single ions from liquid into vacuum from the nanoscale orifice of a charged needle. I will also describe some fundamental aspects of fluid mechanics that we have encountered as we pushed fluidic technologies down to the nanometer scale.

Wednesday, March 28, 2018
2:30 – 3:30 pm
Location – Mason 107
Host: Professor Juan de la Mora

Refreshments served at 2:15 pm