Capillary Liquid Chromatography at Ultra-High Pressure

Time: Wednesday, October 11, 2017 - 10:30am - 11:30am
Type: Seminar Series
Presenter: James W. Jorgenson; Dept. of Chemistry, University of North Carolina at Chapel Hill
Room/Office: Becton 035
Becton Seminar Room
15 Prospect Street
New Haven, CT 06511
United States

Yale School of Engineering & Applied Science Department of Chemical & Environmental Engineering
and The Goizueta Foundation present the Csaba Horváth Distinguished Lecture in Chemical Engineering

James W. Jorgenson
Department of Chemistry
University of North Carolina at Chapel Hill

"Capillary Liquid Chromatography At Ultra-High Pressure"

Abstract: With operating pressures in the range of 1,000 to 3,000 bar in Ultra High Pressure Liquid Chromatography (UHPLC), particles around 1 micron size should be close to optimum for separations of small molecules. Larger molecules such as peptides and proteins should bene t from even further reductions in particle size. We are beginning to determine some of the underlying structural causes of poor column e ciency, and have discovered how to pack e cient capillary columns with porous silica-based reversed phase particles down to 1 micron diameter. Methods for the slurry packing of micron-sized particles will be described. The correlation of several characteristics of the packed bed structure with column performance will be discussed.

We have also recently become interested in porous graphitic carbon as an alternative stationary phase to silica-based reversed phase for lower molecular weight substances, especially some of the more polar metabolites encountered in metabolomics. Graphitic carbon exhibits extraordinary levels of retention for polar organic compounds, which enables the preconcentration of these compounds on-column, as well as improving their prospects for separation. Preliminary results on the use of porous graphitic carbon in capillary LC will be described.

Bio: James Jorgenson was born in Kenosha, Wisconsin in 1952. He received his undergraduate education at Northern Illinois University where he received a B.S. in Chemistry in 1974. Following this he entered graduate school at Indiana University, where he worked in the research group of Professor Milos Novotny, and received a Ph.D. in Chemistry in 1979. His Ph.D. research concerned two principal areas; the identication of mammalian pheromones, and the development of new detection schemes for liquid chromatography.

Dr. Jorgenson joined the faculty of the University of North Carolina as an Assistant Professor of Chemistry in 1979. He was promoted to Associate Professor in 1985, Professor in 1987, appointed the Francis P. Venable Professor of Chemistry in 1994, and William Rand Kenan, Jr. Distinguished Professor of Chemistry in 1999. He was Chair of the Chemistry Department from 2000 to 2005.

Among the honors he has received are the American Chemical Society Analytical Division Award in Chemical Instrumentation (1992), the Martin Medal of the Chromatographic Society (1992), elected a Fellow of the American Association for the Advancement of Science (1992), the American Chemical Society Award in Chromatography (1993), the Golay Medal (1994), the Eastern Analytical Symposium Award in Separation Science (1995), the Torben Bergman Medal of the Swedish Chemical Society (1996), the Anachem Award (1996), the Dal Nogare Award (1998), the Esselen Award for Chemistry in the Public Interest (2004), the Pittsburgh Conference Analytical Chemistry Award (2005), the American Chemical Society Award in Analytical Chemistry (2007), elected to the American Academy of Arts and Sciences (2007), the Ralph N. Adams Award in Bioanalytical Chemistry (2011), and the Csaba Horvath Memorial Award (2016).

Professor Jorgenson is one of the originators of capillary electrophoresis, with his rst publications on this topic appearing in 1981. His current research interests include ultra-high pressure liquid chromatography, multidimensional separations, and microscale separations coupled to mass spectrometry.

Wednesday, October 11, 2017
10:30 am
Becton Seminar Room MC035
15 Prospect Street (another entrance at 10 Hillhouse Avenue)
New Haven, CT 06520

Host: Daniel Rosner

The Csaba Horváth Lectures are made possible by The Goizueta Foundation via a fund administered by the School of Engineering & Applied Science, Department of Chemical & Environmental Engineering, Yale University.