BME Seminar: High Speed SRS Microscopy Goes Super Resolution & Multiplex for Studying Metabolic Activities

Departments: Biomedical Engineering
Time: Tuesday, March 28, 2023 - 4:00pm - 5:00pm
Type: Seminar Series
Presenter: Prof. Lingyan Shi (Bioengineering, UC San Diego)
Room/Office: Room 220
Location:
10 Hillhouse Avenue New Haven, CT 06511
United States
See map: Google Maps

Speaker: Prof. Lingyan Shi (Bioengineering, UC San Diego)

Title: "High Speed SRS Microscopy Goes Super Resolution & Multiplex for Studying Metabolic Activities''

Abstract: Stimulated Raman scattering microscopy (SRS) have revolutionized modern biomedical science and technology. It directly visualizes chemical bonds of biomolecules with specific Raman spectrum profiles. The video-rate imaging speed, molecular selectivity, and high spatial resolution are making an increasingly broad impact in biomedical applications, especially metabolic related diseases. In this talk we will introduce the physical principle of Label free SRS and isotope-probed SRS for imaging metabolic dynamics in living organisms. Then we will discuss how it has been developed and applied in research areas such as aging and diseases, including (1) bioorthogonal metabolic imaging with heavy water (DO-SRS platform), glucose (STRIDE platform), and acetate/glutamine (on-going), for studying metabolic heterogeneity in cells and tissues under physiological/pathological conditions, (2) A-POD enhanced super resolution SRS for visualizing lipid droplet metabolic dynamics in brain under different diet, and (3) multiple molecular detection with PRM-SRS for studying kidney and neurodegenerative diseases.

In-person Location: DL 220 (10 Hillhouse Avenue)

Zoom link: https://yale.zoom.us/j/99108889050